Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-3-11
pubmed:databankReference
pubmed:abstractText
Autophagy, an intrinsically nonselective process, can also target selective cargo for degradation. The mechanism of selective peroxisome turnover by autophagy-related processes (pexophagy), termed micropexophagy and macropexophagy, is unknown. We show how a Pichia pastoris protein, PpAtg30, mediates peroxisome selection during pexophagy. It is necessary for pexophagy, but not for other selective and nonselective autophagy-related processes. It localizes at the peroxisome membrane via interaction with peroxins, and during pexophagy it colocalizes transiently at the preautophagosomal structure (PAS) and interacts with the autophagy machinery. PpAtg30 is required for formation of pexophagy intermediates, such as the micropexophagy apparatus (MIPA) and the pexophagosome (Ppg). During pexophagy, PpAtg30 undergoes multiple phosphorylations, at least one of which is required for pexophagy. PpAtg30 overexpression stimulates pexophagy even under peroxisome-induction conditions, impairing peroxisome biogenesis. Therefore, PpAtg30 is a key player in the selection of peroxisomes as cargo and in their delivery to the autophagy machinery for pexophagy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1878-1551
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
365-76
pubmed:dateRevised
2011-2-28
pubmed:meshHeading
pubmed-meshheading:18331717-Autophagy, pubmed-meshheading:18331717-Biological Transport, Active, pubmed-meshheading:18331717-Cytosol, pubmed-meshheading:18331717-Fungal Proteins, pubmed-meshheading:18331717-Genes, Fungal, pubmed-meshheading:18331717-Green Fluorescent Proteins, pubmed-meshheading:18331717-Microscopy, Fluorescence, pubmed-meshheading:18331717-Models, Biological, pubmed-meshheading:18331717-Molecular Sequence Data, pubmed-meshheading:18331717-Mutation, pubmed-meshheading:18331717-PHEX Phosphate Regulating Neutral Endopeptidase, pubmed-meshheading:18331717-Peroxisomes, pubmed-meshheading:18331717-Phagosomes, pubmed-meshheading:18331717-Phosphorylation, pubmed-meshheading:18331717-Pichia, pubmed-meshheading:18331717-Recombinant Fusion Proteins, pubmed-meshheading:18331717-Vacuoles
pubmed:year
2008
pubmed:articleTitle
PpAtg30 tags peroxisomes for turnover by selective autophagy.
pubmed:affiliation
Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, San Diego, CA 92093-0322, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural