Linked Life Data

18331049

Source:http://linkedlifedata.com/resource/pubmed/id/18331049

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-6
pubmed:abstractText
Optimal concentrations of yeast extract, glucose and potassium phosphate in the fermentation medium have been identified for the maximum cutinase production from the fungi Colleotrichum lindemuthianum. A simplex lattice experimental design for mixtures was used to identify concentration ranges that yield maximum production. Three sets of experiments were performed all involving three ingredients. The sets of experiments differ in the number of concentration levels considered. In the first set we consider four concentration levels (i.e., 0%, 33%, 67%, 100%), and in the second and third sets we consider five and six levels, respectively. Results showed that the interactions among the nutrient ingredients are best captured when five- and six-level experiments are carried out. An algorithm has been proposed in this study to design the optimal medium. Various models were also developed to predict the enzyme production, and it is concluded that the cubic model obtained using six-level experimental data gives the best model. The study also highlighted the synergistic interaction between yeast extract and glucose toward cutinase production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1520-6033
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
648-54
pubmed:meshHeading
pubmed:articleTitle
Optimization of the media ingredients for cutinase production from Colleotrichum lindemuthianum using mixture design experiments.
pubmed:affiliation
Department of Mathematics, Dowling College, Idle Hour Blvd., Oakdale, New York 11955, USA.
pubmed:publicationType
Journal Article