Linked Life Data

18318713

Source:http://linkedlifedata.com/resource/pubmed/id/18318713

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-3-12
pubmed:abstractText
Changes in the outer membrane subproteome of Escherichia coli along the transition to the viable but nonculturable state (VBNC) were studied. The VBNC state was triggered by exposure of E. coli cells to adverse conditions such as aquatic systems, starvation, suboptimal temperature, visible light irradiation and seawater. The subproteome, obtained according to Molloy et al., was analysed at the beginning of exposure (inoculum, phase 1), after a variable exposure time (95% of population culturable, phase 2) and when populations were mainly in the VBNC state (95% of cells VBNC, phase 3). Proteome changes were dependent on adverse conditions inducing the transition and were detected mainly in phase 2. The permanence of E. coli cells in seawater under illumination conditions entailed a dramatic rearrangement of the outer membrane subproteome involving 106 new spots, some of which could be identified by peptide fingerprinting. However, proteins exclusive to the VBNC state were not detected.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0168-6496
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-36
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Changes in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable state.
pubmed:affiliation
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Ciencia y Tecnología, Universidad del País Vasco, Bilbao, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't