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rdf:type |
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lifeskim:mentions |
umls-concept:C0013845,
umls-concept:C0017837,
umls-concept:C0025663,
umls-concept:C0035647,
umls-concept:C0085089,
umls-concept:C0162768,
umls-concept:C0872079,
umls-concept:C1167624,
umls-concept:C1548779,
umls-concept:C1947902,
umls-concept:C2603343,
umls-concept:C2827499
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pubmed:issue |
1
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pubmed:dateCreated |
2008-4-8
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pubmed:abstractText |
Site-directed spin labeling electron paramagnetic resonance methods have been an important tool in studying protein-protein interactions. Labels are often attached to a cysteine residue, and spectra are acquired with and without binding partner(s) to provide information on the binding. This requires a knowledge of the label location which is simplified if the label remains faithfully attached to the designated residue in the complex. We report a system where this is not the case because the label was extracted by dialysis-resistant glutathione molecules. Once this artifact is identified, spectral subtraction provides a solution for meaningful data interpretation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-10966640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-14661984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-14744249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-16313192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-16402913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-16756495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-16949813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-17239579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-17463080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-17573347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-17606918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-17696498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-6280514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18316034-8672470
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1096-0309
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
376
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
160-2
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Potential artifacts in using a glutathione S-transferase fusion protein system and spin labeling electron paramagnetic resonance methods to study protein-protein interactions.
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pubmed:affiliation |
Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, MC 111, Chicago, IL 60607, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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