Source:http://linkedlifedata.com/resource/pubmed/id/18302930
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2008-3-25
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pubmed:abstractText |
Histamine H3 receptor (H3R), one of G protein-coupled receptors (GPCRs), has been known to regulate neurotransmitter release negatively in central and peripheral nervous systems. Recently, a variety of intracellular proteins have been identified to interact with carboxy (C)-termini of GPCRs, and control their intracellular trafficking and signal transduction efficiencies. Screening for such proteins that interact with the C-terminus of H3R resulted in identification of one of the chloride intracellular channel (CLIC) proteins, CLIC4. The association of CLIC4 with H3R was confirmed in in vitro pull-down assays, coimmunoprecipitation from rat brain lysate, and immunofluorescence microscopy of rat cerebellar neurons. The data from flowcytometric analysis, radioligand receptor binding assay, and cell-based ELISA indicated that CLIC4 enhanced cell surface expression of wild-type H3R, but not a mutant form of the receptor that failed to interact with CLIC4. These results indicate that, by binding to the C-terminus of H3R, CLIC4 plays a critical role in regulation of the receptor cell surface expression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1090-2104
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pubmed:author |
pubmed-author:AriakeKyoheiK,
pubmed-author:FukunagaKohjiK,
pubmed-author:HaraguchiMitsuyaM,
pubmed-author:KondoHisatakeH,
pubmed-author:KuramasuAtsuoA,
pubmed-author:MagdeM DMDJr,
pubmed-author:SakagamiHiroyukiH,
pubmed-author:SatoTakeyaT,
pubmed-author:SukegawaJunJ,
pubmed-author:YanagisawaTeruyukiT,
pubmed-author:YanaiKazuhikoK
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pubmed:issnType |
Electronic
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pubmed:day |
2
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pubmed:volume |
369
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
603-8
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pubmed:meshHeading |
pubmed-meshheading:18302930-Animals,
pubmed-meshheading:18302930-CHO Cells,
pubmed-meshheading:18302930-Cell Membrane,
pubmed-meshheading:18302930-Cerebellum,
pubmed-meshheading:18302930-Chloride Channels,
pubmed-meshheading:18302930-Cricetinae,
pubmed-meshheading:18302930-Cricetulus,
pubmed-meshheading:18302930-Neurons,
pubmed-meshheading:18302930-PC12 Cells,
pubmed-meshheading:18302930-Rats,
pubmed-meshheading:18302930-Rats, Wistar,
pubmed-meshheading:18302930-Receptors, Histamine H3
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pubmed:year |
2008
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pubmed:articleTitle |
CLIC4 interacts with histamine H3 receptor and enhances the receptor cell surface expression.
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pubmed:affiliation |
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan.
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pubmed:publicationType |
Journal Article
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