Source:http://linkedlifedata.com/resource/pubmed/id/18299129
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2008-3-19
|
| pubmed:abstractText |
Fish gonadal phenotype is very sensitive to sex steroid and functional masculinizations can be obtained in most species using androgen treatments. To gain insight into the molecular effects of androgen-induced masculinization we characterized, in the rainbow trout, the gonadal expression profiles of 103 candidate genes involved in sex differentiation and early gametogenesis. The androgen treatment (11beta-hydroxyandrostenedione, 10 mg/kg of food for 3 months) was administered in a genetic all-female population. Gonads were sampled at different time points in genetic all-male and all-female control populations and in the androgen-treated group. Gene expression profiles were recorded by real-time RT-PCR and biological samples and gene expressions were compared using a global clustering analysis. This analysis revealed that masculinization with androgens acts firstly by repressing granulosa cell related genes, including genes involved in ovarian differentiation (foxl2a, fst, cyp19a1a), and subsequently by repressing genes important for early oogenesis (gdf9, bcl2lb, fancl, gcl, fshb, lhb, sox23, sox24, nup62 and vtgr). However, this masculinizing treatment did not induce a testicular differentiation similar to what was observed in the control male population. This was especially noticeable for many Leydig cell genes encoding proteins involved in steroidogenesis or its control (hsd3b1, star, cyp17a1, cyp11b2.1 and nr5a1b) that were down-regulated in the androgen-treated group. Concomitantly some Sertoli cells marker genes were up-regulated by the androgen treatment (sox9a.1, nr0b1, cldn11, dmrt1) whereas others were down-regulated (amh, sox9a.2), suggesting a partial differentiation of the Sertoli cell lineage. Overall, this suggests that the crucial step of this masculinization process is the de-differentiation of the granulosa cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0016-6480
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
156
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
369-78
|
| pubmed:meshHeading |
pubmed-meshheading:18299129-Androgens,
pubmed-meshheading:18299129-Animals,
pubmed-meshheading:18299129-Cell Differentiation,
pubmed-meshheading:18299129-DNA Primers,
pubmed-meshheading:18299129-Data Interpretation, Statistical,
pubmed-meshheading:18299129-Female,
pubmed-meshheading:18299129-Gene Expression Profiling,
pubmed-meshheading:18299129-Genetic Markers,
pubmed-meshheading:18299129-Leydig Cells,
pubmed-meshheading:18299129-Male,
pubmed-meshheading:18299129-Multigene Family,
pubmed-meshheading:18299129-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18299129-Oncorhynchus mykiss,
pubmed-meshheading:18299129-Oogenesis,
pubmed-meshheading:18299129-Ovary,
pubmed-meshheading:18299129-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18299129-Sex Differentiation,
pubmed-meshheading:18299129-Testis
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Expression profiling of candidate genes during ovary-to-testis trans-differentiation in rainbow trout masculinized by androgens.
|
| pubmed:affiliation |
INRA, UR1037 SCRIBE, IFR140, Ouest-Genopole, Rennes, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|