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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-8-6
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pubmed:abstractText |
The nit-3 gene of the filamentous fungus Neurospora crassa encodes the enzyme nitrate reductase, which catalyzes the first reductive step in the highly regulated nitrate assimilatory pathway. The nucleotide sequence of nit-3 was determined and translates to a protein of 982 amino acid residues with a molecular weight of approximately 108 kDa. Comparison of the deduced nit-3 protein sequence with the nitrate reductase protein sequences of other fungi and higher plants revealed that a significant amount of homology exists, particularly within the three cofactor-binding domains for molybdenum, heme and FAD. The synthesis and turnover of the nit-3 mRNA were also examined and found to occur rapidly and efficiently under changing metabolic conditions.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
227
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
213-23
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1829499-Amino Acid Sequence,
pubmed-meshheading:1829499-Base Sequence,
pubmed-meshheading:1829499-Blotting, Northern,
pubmed-meshheading:1829499-Flavin-Adenine Dinucleotide,
pubmed-meshheading:1829499-Gene Expression Regulation, Fungal,
pubmed-meshheading:1829499-Genes, Fungal,
pubmed-meshheading:1829499-Heme,
pubmed-meshheading:1829499-Kinetics,
pubmed-meshheading:1829499-Molecular Sequence Data,
pubmed-meshheading:1829499-Molybdenum,
pubmed-meshheading:1829499-Neurospora crassa,
pubmed-meshheading:1829499-Nitrate Reductase,
pubmed-meshheading:1829499-Nitrate Reductases,
pubmed-meshheading:1829499-Nitrates,
pubmed-meshheading:1829499-RNA, Fungal,
pubmed-meshheading:1829499-RNA, Messenger,
pubmed-meshheading:1829499-Restriction Mapping,
pubmed-meshheading:1829499-Sequence Homology, Nucleic Acid
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pubmed:year |
1991
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pubmed:articleTitle |
Nit-3, the structural gene of nitrate reductase in Neurospora crassa: nucleotide sequence and regulation of mRNA synthesis and turnover.
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pubmed:affiliation |
Department of Biochemistry, Ohio State University, Columbus 43210.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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