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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2008-3-31
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pubmed:abstractText |
An improved method is described for culturing primary rat brain capillary endothelial cells (RBCEC) on glass, covered by Matrigel. The procedure using Matrigel yields spindle-shaped endothelial cells exhibiting close cell-cell appositions seen on electron microscopic sections. These cells permanently express tight junction proteins ZO-1, claudin-5 and the adherent junction protein beta-catenin, as revealed by immunofluorescence. Furthermore, glass coverslips covered with Matrigel provide a stable and low-background fluorescent base for microfluorimetric calcium measurements. By this method, hereby we show that the PAR-4 agonist peptide induces transient [Ca2+]i changes with different kinetics compared to that due to activation of the PAR-1 receptor. This indicates that RBCE cells grown on Matrigel express PAR-4 receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/CLDN5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Puromycin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombin,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/matrigel,
http://linkedlifedata.com/resource/pubmed/chemical/protease-activated receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/zonula occludens-1 protein
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0197-0186
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1234-9
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pubmed:meshHeading |
pubmed-meshheading:18294734-Animals,
pubmed-meshheading:18294734-Biocompatible Materials,
pubmed-meshheading:18294734-Calcium,
pubmed-meshheading:18294734-Calcium Signaling,
pubmed-meshheading:18294734-Cell Culture Techniques,
pubmed-meshheading:18294734-Cell Differentiation,
pubmed-meshheading:18294734-Cell Separation,
pubmed-meshheading:18294734-Cells, Cultured,
pubmed-meshheading:18294734-Cerebral Arteries,
pubmed-meshheading:18294734-Collagen,
pubmed-meshheading:18294734-Drug Combinations,
pubmed-meshheading:18294734-Endothelial Cells,
pubmed-meshheading:18294734-Immunohistochemistry,
pubmed-meshheading:18294734-Intercellular Junctions,
pubmed-meshheading:18294734-Laminin,
pubmed-meshheading:18294734-Membrane Proteins,
pubmed-meshheading:18294734-Microscopy, Electron, Transmission,
pubmed-meshheading:18294734-Peptides,
pubmed-meshheading:18294734-Phosphoproteins,
pubmed-meshheading:18294734-Protein Synthesis Inhibitors,
pubmed-meshheading:18294734-Proteoglycans,
pubmed-meshheading:18294734-Puromycin,
pubmed-meshheading:18294734-Rats,
pubmed-meshheading:18294734-Receptors, Thrombin,
pubmed-meshheading:18294734-Time Factors,
pubmed-meshheading:18294734-beta Catenin
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pubmed:year |
2008
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pubmed:articleTitle |
Identification of protease-activated receptor-4 (PAR-4) in puromycin-purified brain capillary endothelial cells cultured on Matrigel.
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pubmed:affiliation |
Department of Medical Biochemistry, Semmelweis University, Neurobiochemical Group, Hungarian Academy of Sciences, Szentagothai Knowledge Center, Budapest, Hungary.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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