Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-7-31
pubmed:abstractText
The product of the rep gene of ColE2 is required for initiation of ColE2 DNA replication. The rep gene was placed under the control of the promoters, PL and PR, and the heat-labile cl857 repressor of bacteriophage lambda. The Rep protein was identified as a 35 Kd protein by the maxicell method in combination with heat-induced expression. The protein was efficiently expressed from these promoters in unirradiated cells and accumulated up to a few per cent of the total cellular proteins. It was partially purified (about 80% pure) and its properties examined. The amino acid sequence of the amino terminal portion of the partially purified protein agreed well with that predicted from the nucleotide sequence of the rep gene. One of the characteristic features of the rep gene is frequent usage of rare codons, especially those for arginine. The protein specifically stimulated replication of ColE2 DNA but not that of ColE3 DNA in crude cell extracts of Escherichia coli. Specific binding of the protein to plasmid DNA containing the origin region of ColE2 was demonstrated by the filter binding method. Neither endonuclease activity nor topoisomerase activity was detected by using ColE2 DNA.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-1100839, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-2651878, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-2693943, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-2780544, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-2841566, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-364941, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-368040, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-383387, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-3892228, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-4595573, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-4902069, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-4915295, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-4922634, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-5340680, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6154869, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6219272, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6236744, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6259124, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6313941, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6340113, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6344017, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6809059, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-6990417, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-787767, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-794878, http://linkedlifedata.com/resource/pubmed/commentcorrection/1829159-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
19
pubmed:geneSymbol
cI857, rep
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2875-80
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Identification of a plasmid-coded protein required for initiation of ColE2 DNA replication.
pubmed:affiliation
Department of Biology, Faculty of Science, Osaka University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't