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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0007765,
umls-concept:C0009017,
umls-concept:C0018437,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0042219,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C1711351,
umls-concept:C2700640
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pubmed:issue |
3
|
|
pubmed:dateCreated |
1991-7-3
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|
pubmed:abstractText |
cDNA for the 16 kDa subunit of vacuolar H(+)-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
176
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1062-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1828149-Adrenal Medulla,
pubmed-meshheading:1828149-Amino Acid Sequence,
pubmed-meshheading:1828149-Animals,
pubmed-meshheading:1828149-Base Sequence,
pubmed-meshheading:1828149-Binding Sites,
pubmed-meshheading:1828149-Cattle,
pubmed-meshheading:1828149-Cerebellum,
pubmed-meshheading:1828149-Cloning, Molecular,
pubmed-meshheading:1828149-DNA,
pubmed-meshheading:1828149-Drosophila,
pubmed-meshheading:1828149-Electric Organ,
pubmed-meshheading:1828149-Escherichia coli,
pubmed-meshheading:1828149-Gene Library,
pubmed-meshheading:1828149-Macromolecular Substances,
pubmed-meshheading:1828149-Mice,
pubmed-meshheading:1828149-Mice, Inbred ICR,
pubmed-meshheading:1828149-Molecular Sequence Data,
pubmed-meshheading:1828149-Molecular Weight,
pubmed-meshheading:1828149-Oligonucleotide Probes,
pubmed-meshheading:1828149-Proton-Translocating ATPases,
pubmed-meshheading:1828149-Restriction Mapping,
pubmed-meshheading:1828149-Saccharomyces cerevisiae,
pubmed-meshheading:1828149-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1828149-Torpedo,
pubmed-meshheading:1828149-Vacuoles
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pubmed:year |
1991
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pubmed:articleTitle |
Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H(+)-ATPase from mouse cerebellum.
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pubmed:affiliation |
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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