Linked Life Data

18274769

Source:http://linkedlifedata.com/resource/pubmed/id/18274769

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-5-6
pubmed:abstractText
We reported previously that Coprinus cinereus Lim15/Dmc1 (CcLim15), a meiosis-specific recA-like protein, could specifically activate C. cinereus DNA topoisomerase II (CcTopII). In particular, it enhanced the catenation activity of CcTopII in vitro at the meiotic prophase stage (Iwabata K, Koshiyama A, Yamaguchi T, Sugawara H, Hamada NF, Namekawa HS, Ishii S, Ishizaki T, Chiku H, Nara T, Sakaguchi K, Nucleic Acids Res, 33:5809-5818, 2005). In this study, the interaction between CcTopII and CcLim15, especially during catenation, was investigated in detail using atomic force microscopy. We demonstrated earlier that CcLim15 enhanced the catenation activity of CcTopII in a dose-dependent manner. When using two different-sized plasmid rings (5.4 and 3 kbp), which did not have any homologous sequence regions, equal proportions of homologous and heterologous catenanes were produced, suggesting that CcLim15 causes an increase in catenation activity irrespective of the presence of homologous sequences between the rings. We also showed that CcLim15 works as a DNA-condensing agent. Therefore, we speculate that CcLim15 may work as a DNA-condensing factor specific to the zygotene event and that CcTopII is likely to resolve tangles when the chromosomes initiate pairing at multiple sites by CcLim15.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0009-5915
pubmed:author
pubmed:issnType
Print
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
297-302
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Lim15/Dmc1 enhances DNA topoisomerase II catenation activity independent of sequence homology.
pubmed:affiliation
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
pubmed:publicationType
Journal Article