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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-2-11
pubmed:abstractText
It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1934-3647
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 15.4
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Molecular cloning of PCR products.
pubmed:affiliation
MJ Research, Watertown, Massachusetts, USA.
pubmed:publicationType
Journal Article