Linked Life Data

18260629

Source:http://linkedlifedata.com/resource/pubmed/id/18260629

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-3-6
pubmed:abstractText
A partially purified protein fraction was isolated from seed flour of the Indian wild bean, Lablab purpureus, by ion exchange and size-exclusion chromatographies. Partially purified L. purpureus proteins had hemagglutination and glycoslyation properties similar to those of lectins or lectin-like proteins from other pulses. Data obtained from two-dimensional gel electrophoresis, MALDI-TOF, and MALDI-TOF/TOF and N-terminal protein sequencing of the isolated polypeptides from L. purpureus demonstrated that the extract contained proteins similar to isoforms of arcelins 3 and 4 and pathogenesis-related protein 1 (PvPR1) of Phaseolus vulgaris. L. purpureus proteins were resistant to degradation by the commercial enzymes trypsin and chymotrypsin and were moderately resistant to pepsin, but were readily hydrolyzed to smaller peptides by papain. Insect feeding bioassays of the extract with the storage pests Rhyzopertha dominica and Oryzaephilus surinamensis, internal and external feeders of grain, respectively, demonstrated that L. purpureus proteins at 2% in the diet resulted in retarded development. However, a 5% dose of the L. purpureus fraction resulted in complete mortality of all larvae in both species. This study has demonstrated that proteins in the partially purified L. purpureus extract have the potential to control storage pests in cereals transformed with L. purpureus defense-related genes, but the need for more studies regarding efficacy and safety is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1676-82
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Arcelins from an Indian wild pulse, Lablab purpureus, and insecticidal activity in storage pests.
pubmed:affiliation
Thiagarajar Center for Entomological Science, Department of Zoology, Thiagarajar College (Autonomous), Madurai 625009, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't