Linked Life Data

18257392

Source:http://linkedlifedata.com/resource/pubmed/id/18257392

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-8
pubmed:abstractText
The large zinc finger proteins, ZAS, regulate the transcription of a variety of genes involved in cell growth, development, and metastasis. They also function in the signal transduction of the TGF-beta and TNF-alpha pathways. However, the endogenous protein of a representative member, ZAS3, is rapidly degraded in primary lymphocytes, which limits the determination of its physiological function in vitro. Therefore, we have generated mice with targeted disruption of ZAS3. Oligonucleotide-based microarray analyses revealed subtle but consistent differences in the expression of genes, many of which are associated with receptor or signal transduction activities between ZAS3+/+ and ZAS3-/- thymi. Gel mobility shift assays showed altered DNA binding activities of NF-kappaB and AP-1 proteins in ZAS3-deficient tissues, including the thymus. Lymphocyte analysis suggested a subtle but broad function of ZAS3 in regulating T-cell development and activation. In CD3+ ZAS3-/- thymocytes, the CD4/ CD8 ratio was decreased and CD69 expression was decreased. In peripheral CD4+ ZAS3-/- lymphocytes we observed an increased number of memory T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1052-2166
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-100
pubmed:meshHeading
pubmed-meshheading:18257392-Animals, pubmed-meshheading:18257392-Base Sequence, pubmed-meshheading:18257392-Blotting, Southern, pubmed-meshheading:18257392-Cell Proliferation, pubmed-meshheading:18257392-DNA, pubmed-meshheading:18257392-DNA Primers, pubmed-meshheading:18257392-DNA-Binding Proteins, pubmed-meshheading:18257392-Electrophoretic Mobility Shift Assay, pubmed-meshheading:18257392-Mice, pubmed-meshheading:18257392-Mice, Inbred C57BL, pubmed-meshheading:18257392-Mice, Knockout, pubmed-meshheading:18257392-NF-kappa B, pubmed-meshheading:18257392-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:18257392-Polymerase Chain Reaction, pubmed-meshheading:18257392-T-Lymphocytes, pubmed-meshheading:18257392-Transcription Factor AP-1, pubmed-meshheading:18257392-Transcription Factors
pubmed:year
2007
pubmed:articleTitle
Disruption of ZAS3 in mice alters NF-kappaB and AP-1 DNA binding and T-cell development.
pubmed:affiliation
Department of Pediatrics and Center for Cell and Developmental Biology, Columbus Children's Research Institute, Columbus, OH 43205, USA.
pubmed:publicationType
Journal Article