Linked Life Data

18257242

Source:http://linkedlifedata.com/resource/pubmed/id/18257242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-2-8
pubmed:databankReference
pubmed:abstractText
Total RNA was isolated from kidney of BaMei pig, a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5alpha, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp, is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25 kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1091-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[Cloning and sequence analysis of SOCS-2 gene in pig].
pubmed:affiliation
Northwest A&F University, Laboratory of Animal Fat Deposition and Muscle Development, Yang Ling 712100, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't