Linked Life Data

1824852

Source:http://linkedlifedata.com/resource/pubmed/id/1824852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-2-25
pubmed:abstractText
Mast cell-fibroblast interactions have been extensively investigated in the last few years. Fibroblasts support the in vitro survival but not proliferation of mouse connective-tissue type mast cells. However, the factor(s) that allow their survival on fibroblast monolayers has not been identified. We have investigated the presence of mRNA for IL-3 and granulocyte-macrophage-CSF in single mouse mast cells, before and after co-culture with 3T3 fibroblasts, using the polymerase chain reaction technique. The system was calibrated first by using in vitro generated population of mouse bone-marrow derived mast cells (BMMC). Significant differences in the amplification of IL-3 cDNA were observed in each of the BMMC cells examined, whereas the amplification of cDNA for the alpha-subunit of the Fc epsilon RI were similar. Inasmuch as murine cultured IL-3-dependent mast cells differentiate into connective tissue-like mast cells when co-cultured with 3T3 fibroblasts without any exogenous supply of growth factors, it was of interest to determine whether these connective tissue-like mast cells produce IL-3 message. Separation of the differentiated BMMC from the fibroblast monolayer, by either trypsinization or by single cell manipulation revealed the synthesis of a detectable amount of IL-3 mRNA in these mast cells. Whether this IL-3 mRNA was induced by fibroblasts was further investigated using connective tissue mast cells freshly purified from the mouse peritoneal cavity. Only about 20% of these connective tissue mast cells produced detectable amount of granulocyte-macrophage-CSF mRNA whereas in less than 10% of the cells IL-3 mRNA was detected. However, when these connective tissue mast cells were co-cultured with 3T3 fibroblasts for 18 hours and then separated, IL-3 mRNA were detected in most of the cells whereas no such mRNA was detected in tissue mast cells incubated for 18 h with medium derived from 3T3 fibroblasts. Therefore we conclude that fibroblasts induce the accumulation of IL-3 mRNA in connective tissue mast cells. The production of IL-3 may play a role in the survival of this type of mast cells on the fibroblast monolayer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
981-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1824852-Animals, pubmed-meshheading:1824852-Antigens, Differentiation, pubmed-meshheading:1824852-Cell Communication, pubmed-meshheading:1824852-Cells, Cultured, pubmed-meshheading:1824852-Connective Tissue Cells, pubmed-meshheading:1824852-Female, pubmed-meshheading:1824852-Fibroblasts, pubmed-meshheading:1824852-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:1824852-Humans, pubmed-meshheading:1824852-Interleukin-3, pubmed-meshheading:1824852-Mast Cells, pubmed-meshheading:1824852-Mice, pubmed-meshheading:1824852-Mice, Inbred C57BL, pubmed-meshheading:1824852-Mice, Inbred DBA, pubmed-meshheading:1824852-Polymerase Chain Reaction, pubmed-meshheading:1824852-RNA, Messenger, pubmed-meshheading:1824852-Receptors, Fc, pubmed-meshheading:1824852-Receptors, IgG
pubmed:year
1991
pubmed:articleTitle
Connective tissue mast cells in contact with fibroblasts express IL-3 mRNA. Analysis of single cells by polymerase chain reaction.
pubmed:affiliation
Institute of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't