Linked Life Data

18218331

Source:http://linkedlifedata.com/resource/pubmed/id/18218331

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-6-9
pubmed:abstractText
Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contribute to stabilizing resting membrane potential, thus controlling neuron excitability. Subclasses of nociceptive neurons differ in their excitability, therefore, these channels could be a distinguishing marker. We investigated isolated dorsal root ganglion neurons from a non-rodent species, the pig, Sus scrofa domesticus. Single labeling revealed capsaicin-induced cobalt-uptake in 54.3% and transient receptor potential V1 (TRPV1) immunoreactivity in 55.1% of all neurons. Ruthenium red and capsazepine suppressed capsaicin-induced cobalt-uptake. HCN-1 and HCN-2 channel isoform immunoreactivity was detected in 82.6% and 88.3%, respectively, and binding of IB4 in 29.4% of all neurons. Double labeling revealed that out of the capsaicin-positive neurons, 42.3% were IB4-positive, 80.0% immunoreactive for the HCN-1, and 77.3% for the HCN-2 channel isoform, respectively. Neurons lacking HCN-1 or HCN-2 channel isoforms were mostly capsaicin-positive and IB4-negative. The soma size of neurons lacking HCN-1 and/or HCN-2 channels was small to medium. Western blot analysis showed protein products of sizes similar to those of HCN-1 and HCN-2 channel isoforms. Functionally, in patch-clamp experiments, some neurons were unresponsive to membrane hyperpolarization, thus, probably lacking HCN channels. In conclusion, in porcine dorsal root ganglion neurons there is a subset of capsaicin-positive, IB4-negative neurons lacking HCN-1 and/or HCN-2 channel isoforms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1532-2149
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
775-89
pubmed:meshHeading
pubmed-meshheading:18218331-Animals, pubmed-meshheading:18218331-Blotting, Western, pubmed-meshheading:18218331-Capsaicin, pubmed-meshheading:18218331-Cyclic Nucleotide-Gated Cation Channels, pubmed-meshheading:18218331-Female, pubmed-meshheading:18218331-Fluorescent Antibody Technique, pubmed-meshheading:18218331-Ganglia, Spinal, pubmed-meshheading:18218331-Ion Channels, pubmed-meshheading:18218331-Male, pubmed-meshheading:18218331-Membrane Potentials, pubmed-meshheading:18218331-Neurons, Afferent, pubmed-meshheading:18218331-Nociceptors, pubmed-meshheading:18218331-Patch-Clamp Techniques, pubmed-meshheading:18218331-Plant Lectins, pubmed-meshheading:18218331-Potassium Channels, pubmed-meshheading:18218331-Sensory System Agents, pubmed-meshheading:18218331-Sus scrofa, pubmed-meshheading:18218331-TRPV Cation Channels
pubmed:year
2008
pubmed:articleTitle
A subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels.
pubmed:affiliation
Department of Anesthesiology and Intensive Care Medicine, Faculty of Clinical Medicine, University of Heidelberg, Mannheim, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't