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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-25
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pubmed:abstractText |
The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA beta-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of beta-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-25
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pubmed:meshHeading |
pubmed-meshheading:18164806-Animals,
pubmed-meshheading:18164806-Blotting, Western,
pubmed-meshheading:18164806-Cell Line, Tumor,
pubmed-meshheading:18164806-DNA,
pubmed-meshheading:18164806-Drug Delivery Systems,
pubmed-meshheading:18164806-Gene Transfer Techniques,
pubmed-meshheading:18164806-Humans,
pubmed-meshheading:18164806-Lactic Acid,
pubmed-meshheading:18164806-Male,
pubmed-meshheading:18164806-Mice,
pubmed-meshheading:18164806-Mice, Nude,
pubmed-meshheading:18164806-Nanoparticles,
pubmed-meshheading:18164806-Polyethyleneimine,
pubmed-meshheading:18164806-Polyglycolic Acid,
pubmed-meshheading:18164806-Polymers,
pubmed-meshheading:18164806-Prostatic Neoplasms,
pubmed-meshheading:18164806-Ultrasonic Therapy,
pubmed-meshheading:18164806-beta-Galactosidase
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pubmed:year |
2008
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pubmed:articleTitle |
Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo.
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pubmed:affiliation |
Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, TX 77555, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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