Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-6-26
pubmed:abstractText
Spleens from mice immunized with either human von Willebrand factor, albumin or fragment DD from plasminolyzed fibrin were divided into two equal parts. Subsequently, one half of the spleen cells were fused directly with X-63 mouse myeloma cells and the other half fused after freezing and thawing. The results show that hybridomas may successfully be prepared from frozen/thawn spleen cells, but that a higher cell density than with fresh cells is required. The possibility of making use of frozen spleen cell material implies that valuable time and cell material can be saved, and that fusions can be postponed and performed at the time of choice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0272-457X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
761-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Hybridomas can successfully be prepared from frozen/thawn spleen cells.
pubmed:affiliation
Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway.
pubmed:publicationType
Journal Article