Linked Life Data

18082329

Source:http://linkedlifedata.com/resource/pubmed/id/18082329

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-14
pubmed:abstractText
Anatomical and neurochemical studies indicated that the globus pallidus receives serotonergic innervation from raphe nuclei but the membrane effects of 5-HT on globus pallidus neurons are not entirely clear. We address this question by applying whole-cell patch-clamp recordings on globus pallidus neurons in immature rat brain slices. Under current-clamp recording, 5-HT depolarized globus pallidus neurons and increased their firing rate, an action blocked by both 5-HT(4) and 5-HT(7) receptor antagonists and attributable to an increase in cation conductance(s). Further experiments indicated that 5-HT enhanced the hyperpolarization-activated inward conductance which is blocked by 5-HT(7) receptor antagonist. To determine if 5-HT exerts any presynaptic effects on GABAergic and glutamatergic inputs, the actions of 5-HT on synaptic currents were studied. At 10 microM, 5-HT increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) but had no effect on both the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs). However, 5-HT at a higher concentration (50 microM) decreased the frequency but not the amplitude of the mIPSCs, indicating an inhibition of GABA release from the presynaptic terminals. This effect was sensitive to 5-HT(1B) receptor antagonist. In addition to the presynaptic effects on GABAergic neurotransmission, 5-HT at 50 microM had no consistent effects on glutamatergic neurotransmission, significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) in 4 of 11 neurons and decreased the frequency of mEPSCs in 3 of 11 neurons. In conclusion, we found that 5-HT could modulate the excitability of globus pallidus neurons by both pre- and post-synaptic mechanisms. In view of the extensive innervation by globus pallidus neurons on other basal ganglia nuclei, this action of 5-HT originated from the raphe may have a profound effect on the operation of the entire basal ganglia network.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
151
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
439-51
pubmed:meshHeading
pubmed-meshheading:18082329-Animals, pubmed-meshheading:18082329-Cations, pubmed-meshheading:18082329-Electrophysiology, pubmed-meshheading:18082329-Excitatory Postsynaptic Potentials, pubmed-meshheading:18082329-Globus Pallidus, pubmed-meshheading:18082329-Ion Channels, pubmed-meshheading:18082329-Male, pubmed-meshheading:18082329-Neurons, pubmed-meshheading:18082329-Patch-Clamp Techniques, pubmed-meshheading:18082329-Pindolol, pubmed-meshheading:18082329-Rats, pubmed-meshheading:18082329-Rats, Sprague-Dawley, pubmed-meshheading:18082329-Receptors, Presynaptic, pubmed-meshheading:18082329-Receptors, Serotonin, pubmed-meshheading:18082329-Receptors, Serotonin, 5-HT4, pubmed-meshheading:18082329-Serotonin, pubmed-meshheading:18082329-Serotonin Antagonists, pubmed-meshheading:18082329-Synapses, pubmed-meshheading:18082329-Synaptic Transmission
pubmed:year
2008
pubmed:articleTitle
5-HT excites globus pallidus neurons by multiple receptor mechanisms.
pubmed:affiliation
Department of Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't