Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-5
pubmed:databankReference
pubmed:abstractText
Allantoate amidohydrolases (AAHs) hydrolize the ureide allantoate to ureidoglycolate, CO(2), and two molecules of ammonium. Allantoate degradation is required to recycle purine-ring nitrogen in all plants. Tropical legumes additionally transport fixed nitrogen via allantoin and allantoate into the shoot, where it serves as a general nitrogen source. AAHs from Arabidopsis (Arabidopsis thaliana; AtAAH) and from soybean (Glycine max; GmAAH) were cloned, expressed in planta as StrepII-tagged variants, and highly purified from leaf extracts. Both proteins form homodimers and release 2 mol ammonium/mol allantoate. Therefore, they can truly be classified as AAHs. The kinetic constants determined and the half-maximal activation by 2 to 3 microm manganese are consistent with allantoate being the in vivo substrate of manganese-loaded AAHs. The enzymes were strongly inhibited by micromolar concentrations of fluoride as well as by borate, and by millimolar concentrations of L-asparagine and L-aspartate but not D-asparagine. L-Asparagine likely functions as competitive inhibitor. An Ataah T-DNA mutant, unable to grow on allantoin as sole nitrogen source, is rescued by the expression of StrepII-tagged variants of AtAAH and GmAAH, demonstrating that both proteins are functional in vivo. Similarly, an allantoinase (aln) mutant is rescued by a tagged AtAln variant. Fluorescent fusion proteins of allantoinase and both AAHs localize to the endoplasmic reticulum after transient expression and in transgenic plants. These findings demonstrate that after the generation of allantoin in the peroxisome, plant purine degradation continues in the endoplasmic reticulum.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-10820010, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11030550, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11090219, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11161040, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11180943, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11330038, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-11971139, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-12084828, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-12468722, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-12481089, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-12586392, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-12893945, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-14764906, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-15020640, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-15375205, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-15564127, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-15604670, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-15778462, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16040633, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16169966, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16244137, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16317038, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16333637, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16428265, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16462750, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16496096, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16661891, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16664492, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16665292, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16666035, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16669089, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-16669783, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-17362992, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-17466985, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-17487191, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-17594111, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-17720761, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-1910298, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-2651866, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-4314237, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-5328936, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-5413235, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-5415952, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-7503567, http://linkedlifedata.com/resource/pubmed/commentcorrection/18065556-851223
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
418-30
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean.
pubmed:affiliation
Freie Universität Berlin, Institut für Biologie, Abteilung Biochemie der Pflanzen, 14195 Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't