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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0011306,
umls-concept:C0019163,
umls-concept:C0039194,
umls-concept:C0086222,
umls-concept:C0205171,
umls-concept:C0439801,
umls-concept:C0441889,
umls-concept:C0442726,
umls-concept:C1332714,
umls-concept:C1511790,
umls-concept:C1705733,
umls-concept:C1710082
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pubmed:issue |
1-2
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|
pubmed:dateCreated |
2008-1-29
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pubmed:abstractText |
Hepatitis B surface antigen (HBsAg)-specific T cells were analysed in 16 healthy individuals vaccinated against hepatitis B, using optimised protocols. HBsAg-specific IFN-gamma producing CD4(+) T cells were found at a similar low frequency (0.01%) within the naive T cell subset, the central and the effector memory T cell subset. Overall, HBsAg-specific total CD4(+) T cells were detected in approximately 81% of the HBV vaccinees. The use of dendritic cells substantially increased the otherwise low proliferative responses but not the percentage of IFN-gamma producing cells. The analysis of readily detectable CMV-specific CD4(+) T cell responses was used as a positive control and confirmed the adequacy of our assays. T cell responses associated with (in-) adequate hepatitis B vaccination can now be analysed in more detail.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1759
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
330
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18061610-Adult,
pubmed-meshheading:18061610-CD4-Positive T-Lymphocytes,
pubmed-meshheading:18061610-Case-Control Studies,
pubmed-meshheading:18061610-Cell Proliferation,
pubmed-meshheading:18061610-Cells, Cultured,
pubmed-meshheading:18061610-Cytomegalovirus,
pubmed-meshheading:18061610-Dendritic Cells,
pubmed-meshheading:18061610-Female,
pubmed-meshheading:18061610-Flow Cytometry,
pubmed-meshheading:18061610-Follow-Up Studies,
pubmed-meshheading:18061610-Hepatitis B Antibodies,
pubmed-meshheading:18061610-Hepatitis B Surface Antigens,
pubmed-meshheading:18061610-Hepatitis B Vaccines,
pubmed-meshheading:18061610-Humans,
pubmed-meshheading:18061610-Immunization Schedule,
pubmed-meshheading:18061610-Immunologic Memory,
pubmed-meshheading:18061610-Interferon-gamma,
pubmed-meshheading:18061610-Lymphocyte Activation,
pubmed-meshheading:18061610-Male,
pubmed-meshheading:18061610-Middle Aged,
pubmed-meshheading:18061610-Reference Values,
pubmed-meshheading:18061610-T-Lymphocyte Subsets,
pubmed-meshheading:18061610-Time Factors,
pubmed-meshheading:18061610-Vaccination
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pubmed:year |
2008
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pubmed:articleTitle |
Hepatitis B vaccine-specific CD4(+) T cells can be detected and characterised at the single cell level: limited usefulness of dendritic cells as signal enhancers.
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pubmed:affiliation |
Department of Internal Medicine, Division of Nephrology, Erasmus Medical Center, Rotterdam, The Netherlands. n.litjens@erasmusmc.nl
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pubmed:publicationType |
Journal Article
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