Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-5
pubmed:abstractText
Sister chromatid cohesion is established during S phase near the replication fork. However, how DNA replication is coordinated with chromosomal cohesion pathway is largely unknown. Here, we report studies of fission yeast Ctf18, a subunit of the RFC(Ctf18) replication factor C complex, and Chl1, a putative DNA helicase. We show that RFC(Ctf18) is essential in the absence of the Swi1-Swi3 replication fork protection complex required for the S phase stress response. Loss of Ctf18 leads to an increased sensitivity to S phase stressing agents, a decreased level of Cds1 kinase activity, and accumulation of DNA damage during S phase. Ctf18 associates with chromatin during S phase, and it is required for the proper resumption of replication after fork arrest. We also show that chl1Delta is synthetically lethal with ctf18Delta and that a dosage increase of chl1(+) rescues sensitivities of swi1Delta to S phase stressing agents, indicating that Chl1 is involved in the S phase stress response. Finally, we demonstrate that inactivation of Ctf18, Chl1, or Swi1-Swi3 leads to defective centromere cohesion, suggesting the role of these proteins in chromosome segregation. We propose that RFC(Ctf18) and the Swi1-Swi3 complex function in separate and redundant pathways essential for replication fork stabilization to facilitate sister chromatid cohesion in fission yeast.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-17483413, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-17545166, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-2005825, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-2265610, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-6094012, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-7590311, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8019001, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8290356, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8723351, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8833153, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8939806, http://linkedlifedata.com/resource/pubmed/commentcorrection/18045993-8978690, 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