Linked Life Data

18041776

Source:http://linkedlifedata.com/resource/pubmed/id/18041776

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-12-6
pubmed:abstractText
Synaptosomal associated protein of 25 kDa (SNAP-25) is a SNARE component of the exocytotic apparatus involved in the release of neurotransmitter. We used multiple-labeling immunofluorescence, confocal microscopy, and ultrastructural immunocytochemistry to examine the expression of SNAP-25 in excitatory and inhibitory terminals from different rat and human brain areas. Glutamatergic and GABAergic terminals were identified by staining for the vesicular glutamate transporter (vGLUT1), glutamic acid decarboxylase (GAD67), or the vesicular GABA transporter (vGAT). In all examined areas GABAergic terminals did not display detectable levels of SNAP-25, whereas glutamatergic terminals expressed the protein to a variable extent. Codistribution analysis revealed a high colocalization between pixels detecting SNAP-25 labeling and pixels detecting vGLUT1 immunoreactivity. On the contrary, a low degree of pixel colocalization, comparable to that between two unrelated antigens, was detected between SNAP-25 and vGAT, thus suggesting a random overlap of immunofluorescence signals. Our immunofluorescence evidence was supported by ultrastructural data, which clearly confirmed that SNAP-25 was undetectable in GABAergic terminals identified by both their typical morphology and specific staining for GABA. Interestingly, our ultrastructural results confirmed that a subset of glutamatergic synapses do not contain detectable levels of SNAP-25. The present study extends our previous findings obtained in rodent hippocampus and provides evidence that SNAP-25 expression is highly variable between different axon terminals both in rat and human brain. The heterogeneous distribution of SNAP-25 may have important implications not only in relation to the function of the protein as a SNARE but also in the control of network excitability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9967
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
506
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
373-86
pubmed:meshHeading
pubmed-meshheading:18041776-Animals, pubmed-meshheading:18041776-Antibodies, pubmed-meshheading:18041776-Blotting, Western, pubmed-meshheading:18041776-Brain Chemistry, pubmed-meshheading:18041776-Calcium, pubmed-meshheading:18041776-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:18041776-Glutamic Acid, pubmed-meshheading:18041776-Homeostasis, pubmed-meshheading:18041776-Humans, pubmed-meshheading:18041776-Image Processing, Computer-Assisted, pubmed-meshheading:18041776-Immunohistochemistry, pubmed-meshheading:18041776-Mice, pubmed-meshheading:18041776-Microscopy, Confocal, pubmed-meshheading:18041776-Microscopy, Electron, pubmed-meshheading:18041776-Presynaptic Terminals, pubmed-meshheading:18041776-Rats, pubmed-meshheading:18041776-Rats, Sprague-Dawley, pubmed-meshheading:18041776-Synaptosomal-Associated Protein 25, pubmed-meshheading:18041776-Tissue Embedding
pubmed:year
2008
pubmed:articleTitle
Heterogeneous expression of SNAP-25 in rat and human brain.
pubmed:affiliation
Clinical Epileptology and Experimental Neurophysiology Unit, Fondazione IRCCS, Istituto Neurologico C Besta, via Celoria 11, Milano, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't