Linked Life Data

18023195

Source:http://linkedlifedata.com/resource/pubmed/id/18023195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-18
pubmed:abstractText
In this study, we have biochemically characterized the high-stearic sunflower (Helianthus annuus L.) mutant CAS-14. This mutant displays an abnormal fatty acid composition along the length of the seed when grown at high temperatures. Thus, at the expense of oleate and linoleate, CAS-14 seeds present an increasing axial gradient of stearic acid from the embryo to the distal extreme of the seed. The accumulation of oil in this mutant was initially characterized by analysing the incorporation of radiolabelled acetate, a fatty acid synthetic precursor, into the developing seed tissues in vivo. These experiments indicated that the mutant phenotype was associated with a decrease in the soluble stearoyl-acyl carrier protein desaturase (SAD) activity, as later confirmed when assessing this activity in cell-free extracts from developing sunflower kernels. Furthermore, SAD enzyme gene transcription was also examined in this tissue, identifying the coordinated decrease in the transcription of the sad17 and sad6 genes as underlying the decrease in enzyme activity. On the basis of these results and those previously obtained on the inheritance of the CAS-14 trait, we discuss the possible regulatory mechanisms acting on plant soluble desaturases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0981-9428
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-16
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The biochemical characterization of a high-stearic acid sunflower mutant reveals the coordinated regulation of stearoyl-acyl carrier protein desaturases.
pubmed:affiliation
Instituto de la Grasa (CSIC), Av. Padre García Tejero 4, 41012 Sevilla, Spain. jjsalas@cica.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't