Linked Life Data

1799207

Source:http://linkedlifedata.com/resource/pubmed/id/1799207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-4-13
pubmed:abstractText
The enhancement of fluorescence of the DNA analog poly(d epsilon A) following nucleolytic degradation to mononucleotides was found to be a convenient signal for studying nuclease, especially exonuclease, activity. This measurement, which is simple to obtain and extremely sensitive, detects various kinds of DNases and can be applied to the detection of nucleases in the course of protein purification. The signal change can be observed continuously during the reaction and easily converted to the amount of liberated mononucleotide. The method is thus suitable for quantitative and kinetic studies of exonuclease activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
198
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
246-9
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Use of a fluorescent DNA analog for fluorometric detection of DNase activity.
pubmed:affiliation
Groupe de Cancérogénèse et Mutagénèse Moléculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
pubmed:publicationType
Journal Article