Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-7
pubmed:abstractText
Long-distance regulatory elements and local chromatin structure are critical for proper regulation of gene expression. Here we characterize the chromatin conformation of the chicken alpha-globin silencer-enhancer elements located 3' of the domain. We found a characteristic and erythrocyte-specific structure between the previously defined silencer and the enhancer, defined by two nuclease hypersensitive sites, which appear when the enhancer is active during erythroid differentiation. Fine mapping of these sites demonstrates the absence of a positioned nucleosome and the association of GATA-1. Functional analyses of episomal vectors, as well as stably integrated constructs, revealed that GATA-1 plays a major role in defining both the chromatin structure and the enhancer activity. We detected a progressive enrichment of histone acetylation on critical enhancer nuclear factor binding sites, in correlation with the formation of an apparent nucleosome-free region. On the basis of these results, we propose that the local chromatin structure of the chicken alpha-globin enhancer plays a central role in its capacity to differentially regulate alpha-globin gene expression during erythroid differentiation and development.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-10406808, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-10521391, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-10525404, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-11331588, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-11517325, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-11864602, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-12193659, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-12851470, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15114340, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15136563, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15215894, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15371553, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15659837, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15694345, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15775975, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15809261, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15887235, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15913647, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-15920471, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16039585, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16094312, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16286657, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16614224, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16862119, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-16908533, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-17196618, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-17277777, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-17380126, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-17720709, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-227607, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-3536481, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-6954007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-8100790, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-8168481, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-8563756, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-8665857, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-9641976, http://linkedlifedata.com/resource/pubmed/commentcorrection/17984219-9679020
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1098-5549
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
575-86
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:17984219-Adaptor Proteins, Signal Transducing, pubmed-meshheading:17984219-Animals, pubmed-meshheading:17984219-Cell Differentiation, pubmed-meshheading:17984219-Cell Line, pubmed-meshheading:17984219-Chickens, pubmed-meshheading:17984219-Chromatin, pubmed-meshheading:17984219-Chromatin Assembly and Disassembly, pubmed-meshheading:17984219-Deoxyribonuclease I, pubmed-meshheading:17984219-Enhancer Elements, Genetic, pubmed-meshheading:17984219-Erythroid Cells, pubmed-meshheading:17984219-GATA1 Transcription Factor, pubmed-meshheading:17984219-Globins, pubmed-meshheading:17984219-Histones, pubmed-meshheading:17984219-Mutation, pubmed-meshheading:17984219-Nucleosomes, pubmed-meshheading:17984219-Protein Binding, pubmed-meshheading:17984219-Protein Processing, Post-Translational
pubmed:year
2008
pubmed:articleTitle
GATA-1 modulates the chromatin structure and activity of the chicken alpha-globin 3' enhancer.
pubmed:affiliation
Instituto de Fisiología Celular, Departamento de Genética Molecular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, México D.F. 04510, México. .
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't