Linked Life Data

17981566

Source:http://linkedlifedata.com/resource/pubmed/id/17981566

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-11-5
pubmed:abstractText
MT-SP1 is a type II transmembrane serine protease implicated in a range of human cancers including those of the breast, cervix, ovaries, prostate, colon and gastrointestinal tract. Mouse models have shown it to be critical for proper epidermal development and postnatal survival. However, the role of this enzyme in normal and malignant biology has not yet been fully elucidated. Several groups have identified putative substrates of MT-SP1 in an effort to understand the possible biological processes in which this protease may be involved. Methods for substrate identification include comparing known protein cleavage sequences with MT-SP1 specificity data, in vitro cleavage assays, examining genetic microarrays for enzyme/substrate coexpression, immunohistochemistry for colocalization, and a variety of phenotypic observations using cell culture and mouse models. Given the inherent limitations of each individual method, substrate plausibility is best substantiated using a combination of experimental approaches. Here we review MT-SP1 substrates identified to date and the possible physiological implications of substrate cleavage in cell-microenvironment interactions. This data indicates that MT-SP1 is capable of playing roles in growth factor activation, receptor activation and inactivation, protease activation, and ectodomain shedding. We also present for the first time vascular endothelial growth factor receptor 2 (VEGFR-2) as a putative substrate for MT-SP1.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1093-4715
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
528-39
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17981566-Endothelium, Vascular, pubmed-meshheading:17981566-Enzyme Activation, pubmed-meshheading:17981566-Gene Expression Regulation, pubmed-meshheading:17981566-Humans, pubmed-meshheading:17981566-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:17981566-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:17981566-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:17981566-Neoplasms, pubmed-meshheading:17981566-Peptide Hydrolases, pubmed-meshheading:17981566-Protein Binding, pubmed-meshheading:17981566-Protein Conformation, pubmed-meshheading:17981566-Protein Structure, Tertiary, pubmed-meshheading:17981566-Recombinant Proteins, pubmed-meshheading:17981566-Serine Endopeptidases, pubmed-meshheading:17981566-Vascular Endothelial Growth Factor Receptor-2
pubmed:year
2008
pubmed:articleTitle
MT-SP1 proteolysis and regulation of cell-microenvironment interactions.
pubmed:affiliation
Graduate Program in Biophysics, University of California, San Francisco, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural