Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-11-7
pubmed:abstractText
Peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear receptor regulated by the insulin-sensitizing thiazolidinediones (TZDs). We studied selective modulation of endogenous genes by PPARgamma ligands using microarray, RNA expression kinetics, and chromatin immunoprecipitation (ChIP) in 3T3-L1 adipocytes. We found over 300 genes that were significantly regulated the TZDs pioglitazone, rosiglitazone, and troglitazone. TZD-mediated expression profiles were unique but overlapping. Ninety-one genes were commonly regulated by all three ligands. TZD time course and dose-response studies revealed gene- and TZD-specific expression kinetics. PEPCK expression was induced rapidly but PDK4 expression was induced gradually. Troglitazone EC50 values for PEPCK, PDK4, and RGS2 regulation were greater than those for pioglitazone and rosiglitazone. TZDs differentially induced histone acetylation of and PPARgamma recruitment to target gene promoters. Selective modulation of PPARgamma by TZDs resulted in distinct expression profiles and transcription kinetics which may be due to differential promoter activation and chromatin remodeling of target genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-10214942, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-10490615, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-10748014, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-10871190, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-10953021, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11042466, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11043571, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11095972, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11181544, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11278270, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11416043, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11679588, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11684010, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11756653, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11818508, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-11819031, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-12150997, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-12357248, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-12502716, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-12529412, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-12554792, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-14726447, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-14739072, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-15217816, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-15731109, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-15980550, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-9454824, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-9843961, http://linkedlifedata.com/resource/pubmed/commentcorrection/17963725-9933587
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1090-2104
pubmed:author
pubmed:issnType
Electronic
pubmed:day
21
pubmed:volume
364
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
515-21
pubmed:dateRevised
2011-4-5
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Selective modulation of promoter recruitment and transcriptional activity of PPARgamma.
pubmed:affiliation
Department of Medicine, University of California, San Diego, 9500 Gilman Drive, MC0673, La Jolla, CA 92093, USA. dsears@ucsd.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural