Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2007-10-12
pubmed:abstractText
We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion pumps. Using light from lasers instead of broadband sources as transmittance probe beams can be advantageous when examining optically dense, highly scattering samples such as concentrated microbial cultures. Such a laser-based approach may prove useful in shipboard studies for identifying proteorhodopsin in whole cell suspensions concentrated from seawater.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-6935
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7329-36
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton.
pubmed:affiliation
College of Oceanic & Atmospheric Sciences, Oregon State University, Corvallis, Oregon 97331, USA. desi@coas.oregonstate.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't