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rdf:type |
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lifeskim:mentions |
umls-concept:C0022702,
umls-concept:C0030685,
umls-concept:C0063592,
umls-concept:C0205171,
umls-concept:C0391871,
umls-concept:C0439799,
umls-concept:C0439831,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1963578
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pubmed:issue |
11
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pubmed:dateCreated |
2007-11-1
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pubmed:abstractText |
The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses of the channel to its primary ligands, InsP(3) and Ca(2+), which activate and inhibit channel gating. Here, we describe the kinetic responses of single InsP(3)R channels in native endoplasmic reticulum membrane to rapid ligand concentration changes with millisecond resolution, using a new patch-clamp configuration. The kinetics of channel activation and deactivation showed novel Ca(2+) regulation and unexpected ligand cooperativity. The kinetics of Ca(2+)-mediated channel inhibition showed the single-channel bases for fundamental Ca(2+) release events and Ca(2+) release refractory periods. These results provide new insights into the channel regulatory mechanisms that contribute to complex spatial and temporal features of intracellular Ca(2+) signals.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-10531009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-11099353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-14513550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-14581584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-15003850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-15950055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-16533853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-1663251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-16644799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-17429043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-17526578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-2011747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-2296584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-2339124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-2447968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-2783316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-6281357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-7932238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-7937840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8189206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8262009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8292360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8381210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8558468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8770200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-8770202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9006903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9080360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9210378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9490868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9681192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17932510-9708988
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1469-221X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1044-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17932510-Animals,
pubmed-meshheading:17932510-Calcium,
pubmed-meshheading:17932510-Calcium Channels,
pubmed-meshheading:17932510-Cell Line,
pubmed-meshheading:17932510-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:17932510-Insects,
pubmed-meshheading:17932510-Ion Channel Gating,
pubmed-meshheading:17932510-Ligands,
pubmed-meshheading:17932510-Patch-Clamp Techniques
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pubmed:year |
2007
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pubmed:articleTitle |
Rapid ligand-regulated gating kinetics of single inositol 1,4,5-trisphosphate receptor Ca2+ release channels.
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pubmed:affiliation |
Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. dmak@mail.med.upenn.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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