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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4242
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pubmed:dateCreated |
1976-8-2
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pubmed:abstractText |
The hamster ductus deferens cloned tumor cell line (DDT1) contains a complex steroid receptor protein that binds 3H-labeled 5 alpha-dihydrotestosterone,. Diethylaminoethyl (DEAE) chromatography of cytosol from these cells yields two major receptor peaks of activity. Identification of this steroid binding protein as a cytoplasmic receptor was confirmed by salt dissociation on sucrose gradients, stability of the hormone-receptor complex at 0 degrees C, and the retention patterns on phosphocellulose and DEAE cellulose. Multiple forms of the receptor exist in a single homogeneous cell type. The data support the theory that steroid hormones bind to a cytoplasmic protein receptor composed of dissimilar subunits as the initial step in steroid hormone action.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
192
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
898-900
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:179138-Carrier Proteins,
pubmed-meshheading:179138-Cell Fractionation,
pubmed-meshheading:179138-Cell Line,
pubmed-meshheading:179138-Centrifugation, Density Gradient,
pubmed-meshheading:179138-Chromatography, Affinity,
pubmed-meshheading:179138-Chromatography, Ion Exchange,
pubmed-meshheading:179138-Cyproterone,
pubmed-meshheading:179138-Dihydrotestosterone,
pubmed-meshheading:179138-Estradiol,
pubmed-meshheading:179138-Leiomyosarcoma,
pubmed-meshheading:179138-Male,
pubmed-meshheading:179138-Molecular Weight,
pubmed-meshheading:179138-Neoplasms, Experimental,
pubmed-meshheading:179138-Progesterone,
pubmed-meshheading:179138-Protein Conformation,
pubmed-meshheading:179138-Receptors, Cell Surface,
pubmed-meshheading:179138-Vas Deferens
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pubmed:year |
1976
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pubmed:articleTitle |
Characterization of the androgen receptor from a Syrian hamster ductus deferens tumor cell line (DDT1).
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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