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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
2007-10-3
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pubmed:abstractText |
We have implemented the recently demonstrated technique of chirped-pulse upconversion of midinfrared femtosecond pulses into the visible in a visible pump-midinfrared probe experiment for high-resolution, high-sensitivity measurements over a broad spectral range. We have succeeded in time-resolving the CO ligand transfer process from the heme Fe to the neighboring Cu(B) atom in the bimetallic active site of mammalian cytochrome c oxidase, which was known to proceed in <1 ps, using the full CO vibrational signature of Fe-CO bond breaking and Cu(B)-CO bond formation. Our differential transmission results show a delayed onset of the appearance of the Cu(B)-bound species (200 fs), followed by a 450-fs exponential rise. Trajectories calculated by using molecular-dynamics simulations with a Morse potential for the Cu(B)-C interaction display a similar behavior. Both experimental and calculated data strongly suggest a ballistic contribution to the transfer process.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-10440381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-10490029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-10588704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-10592235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-11325737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-11590135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-11796222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-12010067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-12817148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-14971914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-15212544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-15319472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-15534221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-15839666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-15945144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-16037213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-16135567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-16936881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-17015839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-17261808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-2018766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-2554314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-2843526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-3020018,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-9380672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17895387-9624044
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15705-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17895387-Binding Sites,
pubmed-meshheading:17895387-Carbon Monoxide,
pubmed-meshheading:17895387-Computer Simulation,
pubmed-meshheading:17895387-Deuterium Oxide,
pubmed-meshheading:17895387-Electrochemistry,
pubmed-meshheading:17895387-Electron Transport Complex IV,
pubmed-meshheading:17895387-Heme,
pubmed-meshheading:17895387-Hydrogen Bonding,
pubmed-meshheading:17895387-Kinetics,
pubmed-meshheading:17895387-Ligands,
pubmed-meshheading:17895387-Protein Binding,
pubmed-meshheading:17895387-Spectrophotometry, Infrared
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pubmed:year |
2007
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pubmed:articleTitle |
Direct observation of ligand transfer and bond formation in cytochrome c oxidase by using mid-infrared chirped-pulse upconversion.
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pubmed:affiliation |
Laboratoire d'Optique et Biosciences, Ecole Polytechnique, Centre National de la Recherche Scientifique and Institut National de la Santé et de la Recherche Médicale U696, 91128 Palaiseau, France.
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pubmed:publicationType |
Journal Article
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