Source:http://linkedlifedata.com/resource/pubmed/id/17886186
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2007-9-21
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pubmed:abstractText |
Black aspergilli, and particularly Aspergillus carbonarius, are responsible for ochratoxin A production in grapes. Correct identification of these species is essential for toxicological risk assessment in grape and wine. A low-complexity oligonucleotide microarray (OLISA, Apibio, F) based on DNA oligonucleotides probes, obtained from sequences of the calmodulin gene, was set up in order to detect A. carbonarius, A. japonicus/A. aculeatus and A. ibericus isolated from grape. The designed microarray distinguished all Aspergillus species and the detection limit for A. carbonarius was 3.2 pg of DNA as a template for the PCR reaction. This microarray offers a quick and parallel analysis to detect individual Aspergillus species in pure cultures and in naturally contaminated grape samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0265-203X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1138-47
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pubmed:meshHeading |
pubmed-meshheading:17886186-Aspergillus,
pubmed-meshheading:17886186-DNA, Fungal,
pubmed-meshheading:17886186-Food Contamination,
pubmed-meshheading:17886186-Food Microbiology,
pubmed-meshheading:17886186-Gene Expression,
pubmed-meshheading:17886186-Ochratoxins,
pubmed-meshheading:17886186-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17886186-Polymerase Chain Reaction,
pubmed-meshheading:17886186-Species Specificity,
pubmed-meshheading:17886186-Vitis
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pubmed:year |
2007
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pubmed:articleTitle |
Detection of Aspergillus carbonarius and other black aspergilli from grapes by DNA OLISA microarray.
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pubmed:affiliation |
BioMerieux-Molecular Biology Unit, Parc Polytec, 5 rue des Berges, F-38024 Grenoble cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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