Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2007-11-12
pubmed:abstractText
Recent studies have demonstrated that influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by binding of influenza NS1 protein to the p85 regulatory subunit of PI3K. Our previous study proposed that two polyproline motifs in NS1 (amino acids 164 to 167 [PXXP], SH3 binding motif 1, and amino acids 213 to 216 [PPXXP], SH3 binding motif 2) may mediate binding to the p85 subunit of PI3K. Here we performed individual mutational analyses on these two motifs and demonstrated that SH3 binding motif 1 contributes to the interactions of NS1 with p85beta, whereas SH3 binding motif 2 is not required for this process. Mutant viruses carrying NS1 with mutations in SH3 binding motif 1 failed to interact with p85beta and induce the subsequent activation of PI3K/Akt pathway. Mutant virus bearing mutations in SH3 binding motif 2 exhibited similar phenotype as the wild-type (WT) virus. Furthermore, viruses with mutations in SH3 binding motif 1 induced more severe apoptosis than did the WT virus. Our data suggest that SH3 binding motif 1 in NS1 protein is required for NS1-p85beta interaction and PI3K/Akt activation. Activation of PI3K/Akt pathway is beneficial for virus replication by inhibiting virus induced apoptosis through phosphorylation of caspase-9.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-10579998, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-10801978, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-10933707, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-11090154, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-11421366, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-12163268, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-1322797, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-14504291, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-14709551, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-15763428, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-16466763, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-16715094, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-16963558, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17079289, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17170431, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17188392, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17229704, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17320139, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17325368, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-17371234, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-1849461, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-291907, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7489502, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7624799, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7680959, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7683653, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7701324, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-7958859, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-8139028, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-8525619, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-8978681, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9005851, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9087425, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9192891, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9812896, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9878611, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9888855, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9971827, http://linkedlifedata.com/resource/pubmed/commentcorrection/17881440-9988280
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12730-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
SH3 binding motif 1 in influenza A virus NS1 protein is essential for PI3K/Akt signaling pathway activation.
pubmed:affiliation
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't