Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2007-10-2
pubmed:abstractText
Natural killer (NK) cell cytotoxicity involves the formation of an activating immunological synapse (IS) between the effector and target cell through which granzymes and perforin contained in lytic granules are delivered to the target cell via exocytosis. Inhibition of nonmuscle myosin II in human NK cells with blebbistatin or ML-9 impaired neither effector-target cell conjugation nor formation of a mature activating NK cell IS (NKIS; formation of an actin ring and polarization of the microtubule-organizing center and cytolytic granules to the center of the ring). However, membrane fusion of lytic granules, granzyme secretion, and NK cell cytotoxicity were all effectively blocked. Specific knockdown of the myosin IIA heavy chain by RNA interference impaired cytotoxicity, membrane fusion of lytic granules, and granzyme secretion. Thus, myosin IIA is required for a critical step between NKIS formation and granule exocytosis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-10722873, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-10881170, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-11266472, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-12177428, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-12512936, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-12630704, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-12637748, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-12802007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-1431558, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-14612578, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-14617807, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-14622600, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-15064761, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-15069078, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-15217342, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-15604012, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-15817676, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-16203869, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-16230631, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-16260137, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-16606694, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-16778144, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-2703279, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-7996204, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-8520575, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-8599969, http://linkedlifedata.com/resource/pubmed/commentcorrection/17875677-9856952
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
204
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2285-91
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Myosin IIA is required for cytolytic granule exocytosis in human NK cells.
pubmed:affiliation
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural