Source:http://linkedlifedata.com/resource/pubmed/id/17854980
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2007-12-18
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pubmed:abstractText |
Real-time myocardial contrast echocardiography (MCE) is a noninvasive perfusion imaging method, whereas technical and resolution problems impair its application in small animals. Hence, we investigated the feasibility of MCE in experimental cardiovascular set-ups involving healthy and infarcted myocardium in rats. Twenty-five male Wistar rats were examined under volatile anesthesia (2.5% isoflurane) with high-resolution conventional 2-D echocardiography (2DE) and real-time MCE (Sonos 7,500 with 15MHz-transducer, Philips Medical Systems, Andover, MA, USA) in short-axis view. Contrast agent (SonoVue, Bracco, Milan, Italy) was infused as a bolus into a sublingual vein. Background-subtracted contrast signal intensity (SI) was measured off-line in six end-systolic segments and fitted to an exponential curve (gamma variate). Derived peak SI was subsequently calculated and compared with wall motion and common functional measured quantities (left ventricular end-diastolic diameter [LVEDD], area shortening [AS]). Recordings were performed before and 14 days after left anterior descending (LAD) ligature. Infarction induced anterior wall motion abnormalities (WMA) in all animals (16 akinetic, 9 hypokinetic), increased LVEDD (9.1 +/- 0.6 vs. 7.9 +/- 0.6 mm, p < 0.001), reduced AS (36.1 +/- 10.0 vs. 59.5 +/- 4.1%, p < 0.001) and reduced anterior segmental SI (0.4 +/- 0.4 dB akinetic / 1.7 +/- 1.7 dB hypokinetic vs. 15.8 +/- 10.9 dB preinfarct, p < 0.001 / p < 0.001). Segmental SI in normokinetic segments remained unchanged. Area at risk (perfusion defect) correlated well with WMA (r = 0.838). These data confirmed high-resolution real-time MCE as a rational tool for assessing myocardial perfusion of Wistar rats. It may therefore be a useful diagnostic tool for in-vivo cardiovascular research in small animals.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0301-5629
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-55
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pubmed:meshHeading |
pubmed-meshheading:17854980-Animals,
pubmed-meshheading:17854980-Contrast Media,
pubmed-meshheading:17854980-Coronary Circulation,
pubmed-meshheading:17854980-Feasibility Studies,
pubmed-meshheading:17854980-Male,
pubmed-meshheading:17854980-Myocardial Infarction,
pubmed-meshheading:17854980-Observer Variation,
pubmed-meshheading:17854980-Phospholipids,
pubmed-meshheading:17854980-Rats,
pubmed-meshheading:17854980-Rats, Wistar,
pubmed-meshheading:17854980-Reproducibility of Results,
pubmed-meshheading:17854980-Sulfur Hexafluoride,
pubmed-meshheading:17854980-Ventricular Function, Left
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pubmed:year |
2008
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pubmed:articleTitle |
Real-time myocardial contrast echocardiography for assessing perfusion and function in healthy and infarcted wistar rats.
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pubmed:affiliation |
Second Medical Clinic, Friedrich Alexander University, Erlangen-Nuremberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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