Linked Life Data

1782760

Source:http://linkedlifedata.com/resource/pubmed/id/1782760

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-3-18
pubmed:abstractText
1. A sensitive and specific radioreceptor assay for measuring prolactin in tissue extracts, culture media and serum has been developed utilizing membrane fractions prepared from either chicken or turkey kidney, and an ovine prolactin standard and tracer. 2. Assay sensitivity was 1.0 +/- 0.1 ng per tube, 50% inhibition of binding occurred with 12.8 +/- 1.2 ng of unlabeled ovine prolactin standard, and intra- and interassay coefficients of variation were 3.9% and 8.8%, respectively. 3. The 47,800 g kidney membrane preparation, which yielded maximum specific binding of 20-40%, offered advantages over current methods including use of an easily available and inexpensive tissue, no need for pretreatment of the donor animal, a high yield of receptor protein, and a simplified method of membrane preparation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0305-0491
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
769-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Radioligand receptor assay for prolactin using chicken and turkey kidney membranes.
pubmed:affiliation
USDA-ARS, Avian Physiology Laboratory, Beltsville Agricultural Research Center, MD 20705.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.