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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1992-3-12
|
| pubmed:abstractText |
Pseudomonas putida P111 was isolated by enrichment culture on 2,5-dichlorobenzoate and was also able to grow on 2-chloro-, 3-chloro-, 4-chloro-, 2,3-dichloro-, 2,4-dichloro-, and 2,3,5-trichlorobenzoates. However, 3,5-dichlorobenzoate completely inhibited growth of P111 on all ortho-substituted benzoates that were tested. When 3,5-dichlorobenzoate was added as a cosubstrate with either 3- or 4-chlorobenzoate, cell yields and chloride release were greater than those observed from growth on either monochlorobenzoate alone. Moreover, resting cells of P111 grown on 4-chlorobenzoate released chloride from 3,5-dichlorobenzoate and produced no identifiable intermediate. In contrast, resting cells grown on 2,5-dichlorobenzoate metabolized 3,5-dichlorobenzoate without release of chloride and accumulated a degradation product, which was identified as 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene on the basis of gas chromatography-mass spectrometry confirmation of its two acid-hydrolyzed products, 3,5- and 2,4-dichlorophenol. Since 3,5-dichlorocatechol was rapidly metabolized by cells grown on 2,5-dichlorobenzoate, it is apparent that 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene is not further metabolized by these cells. Moreover, induction of a functional dihyrodiol dehydrogenase would not be required for growth of P111 on other ortho-chlorobenzoates since the corresponding chlorodihydrodiols produced from a 1,2-dioxygenase attack would spontaneously decompose to the corresponding catechols. In contrast, growth on 3-chloro-, 4-chloro-, or 3,5-dichlorobenzoate requires a functional dihydrodiol dehydrogenase, yet only the two monochlorobenzoates appear to induce for it.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-16346660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2036004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2128010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2403263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2499257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2729993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2735918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-2743216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-3223988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-3662518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-453823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-4850728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-5480101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-6497895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-6745818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1781694-697765
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0099-2240
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3361-6
|
| pubmed:dateRevised |
2010-9-9
|
| pubmed:meshHeading | |
| pubmed:year |
1991
|
| pubmed:articleTitle |
Metabolism of and inhibition by chlorobenzoates in Pseudomonas putida P111.
|
| pubmed:affiliation |
Department of Microbiology, University of Panama, Republic of Panama.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|