| pubmed-article:1774323 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C0008150 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:1774323 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:1774323 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:1774323 | pubmed:dateCreated | 1992-3-3 | lld:pubmed |
| pubmed-article:1774323 | pubmed:abstractText | Specific and sensitive amplification of major outer membrane protein (MOMP) gene DNA sequences of Chlamydia psittaci was achieved in a two-step polymerase chain reaction. First, oligonucleotide primers specific for 5' and 3' nontranslated regulatory regions of the MOMP gene were used in a polymerase chain reaction to amplify a DNA fragment of approximately 1,400 bp. A portion of this DNA fragment was amplified in a second reaction using a degenerate oligonucleotide primer specific for a DNA sequence contained within the 1,400-bp DNA fragment and one of the first-step primers. This method detected 10 cognate chlamydial genomes. C. psittaci MOMP genes from two avian strains and from mammalian serovars 1, 7, and 8 were amplified and analyzed by restriction endonuclease digestion. MOMP genes from mammalian serovars 2 through 6 and 9 and from strains of C. trachomatis and C. pneumoniae could not be amplified. Restriction endonuclease analysis with HaeIII indicated a close relationship between C. psittaci strains of avian and mammalian serovar 1 lineage, while those of mammalian serovars 7 and 8 exhibited distinct restriction patterns. DNA sequences corresponding to the mammalian serovar 1-wild type parakeet MOMP genotype of C. psittaci were detected in two of seven milk samples from cases of bovine mastitis. | lld:pubmed |
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| pubmed-article:1774323 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1774323 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1774323 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1774323 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1774323 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1774323 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:1774323 | pubmed:author | pubmed-author:StorzJJ | lld:pubmed |
| pubmed-article:1774323 | pubmed:author | pubmed-author:KousoulasK... | lld:pubmed |
| pubmed-article:1774323 | pubmed:author | pubmed-author:KaltenboeckBB | lld:pubmed |
| pubmed-article:1774323 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1774323 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:1774323 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1774323 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1774323 | pubmed:pagination | 1969-75 | lld:pubmed |
| pubmed-article:1774323 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1774323 | pubmed:meshHeading | pubmed-meshheading:1774323-... | lld:pubmed |
| pubmed-article:1774323 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1774323 | pubmed:articleTitle | Detection and strain differentiation of Chlamydia psittaci mediated by a two-step polymerase chain reaction. | lld:pubmed |
| pubmed-article:1774323 | pubmed:affiliation | Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803. | lld:pubmed |
| pubmed-article:1774323 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1774323 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1774323 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1774323 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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