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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-3-3
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pubmed:abstractText |
Specific and sensitive amplification of major outer membrane protein (MOMP) gene DNA sequences of Chlamydia psittaci was achieved in a two-step polymerase chain reaction. First, oligonucleotide primers specific for 5' and 3' nontranslated regulatory regions of the MOMP gene were used in a polymerase chain reaction to amplify a DNA fragment of approximately 1,400 bp. A portion of this DNA fragment was amplified in a second reaction using a degenerate oligonucleotide primer specific for a DNA sequence contained within the 1,400-bp DNA fragment and one of the first-step primers. This method detected 10 cognate chlamydial genomes. C. psittaci MOMP genes from two avian strains and from mammalian serovars 1, 7, and 8 were amplified and analyzed by restriction endonuclease digestion. MOMP genes from mammalian serovars 2 through 6 and 9 and from strains of C. trachomatis and C. pneumoniae could not be amplified. Restriction endonuclease analysis with HaeIII indicated a close relationship between C. psittaci strains of avian and mammalian serovar 1 lineage, while those of mammalian serovars 7 and 8 exhibited distinct restriction patterns. DNA sequences corresponding to the mammalian serovar 1-wild type parakeet MOMP genotype of C. psittaci were detected in two of seven milk samples from cases of bovine mastitis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-1691145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2315025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2437035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2462716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2565333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2612883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2707861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2753864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2778059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2813066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2822763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2826336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-2892050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-3040664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-3174659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-3517062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-3525517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-3905618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-4055027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1774323-5686006
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1969-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1774323-Animals,
pubmed-meshheading:1774323-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1774323-Base Sequence,
pubmed-meshheading:1774323-Cattle,
pubmed-meshheading:1774323-Chlamydophila psittaci,
pubmed-meshheading:1774323-DNA, Bacterial,
pubmed-meshheading:1774323-Evaluation Studies as Topic,
pubmed-meshheading:1774323-Female,
pubmed-meshheading:1774323-Genes, Bacterial,
pubmed-meshheading:1774323-Mastitis, Bovine,
pubmed-meshheading:1774323-Molecular Sequence Data,
pubmed-meshheading:1774323-Polymerase Chain Reaction,
pubmed-meshheading:1774323-Psittacosis,
pubmed-meshheading:1774323-Species Specificity
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pubmed:year |
1991
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pubmed:articleTitle |
Detection and strain differentiation of Chlamydia psittaci mediated by a two-step polymerase chain reaction.
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pubmed:affiliation |
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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