Linked Life Data

17720533

Source:http://linkedlifedata.com/resource/pubmed/id/17720533

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-9-24
pubmed:abstractText
One major goal in pain research is to identify novel pain targets. Tissue injury, inflammation, and ischemia are usually accompanied by local tissue acidosis, the degree of associated pain or discomfort well correlated with the magnitude of acidification. Proton-sensing ion channels, transient receptor potential/vanilloid receptor subtype 1, and acid-sensing ion channel 3 are involved in acidosis-linked pain. However, whether recently identified proton-sensing G-protein-coupled receptors (GPCRs) also have some contributions is unclear. Proton-sensing GPCRs, including OGR1, GPR4, G2A, and TDAG8, are fully activated at pH 6.4-6.8 in vitro. To understand whether the proton-sensing GPCRs are expressed in nociceptors, we cloned the four mouse genes and examined their tissue distribution and localization in pain-relevant loci, the dorsal root ganglion (DRG). The OGR1 family members were widely expressed in neuronal and non-neuronal tissues. Their transcripts were expressed in the DRG, and most (75-82%) were present in small-diameter neurons responsible for nociception. Approximately 31-40% of total DRG neurons expressed at least two proton-sensing GPCRs. We have also demonstrated that gene expression of proton-sensing GPCRs is changed in ASIC3 knockout mice. Our finding suggests that proton-sensing GPCRs could have some roles in nociception or in compensation of loss of ASIC3 gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1044-7431
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-210
pubmed:dateRevised
2011-11-4
pubmed:meshHeading
pubmed-meshheading:17720533-Animals, pubmed-meshheading:17720533-Cloning, Molecular, pubmed-meshheading:17720533-Computers, pubmed-meshheading:17720533-Ganglia, Spinal, pubmed-meshheading:17720533-Gene Expression Regulation, pubmed-meshheading:17720533-Hydrogen-Ion Concentration, pubmed-meshheading:17720533-Male, pubmed-meshheading:17720533-Mice, pubmed-meshheading:17720533-Mice, Knockout, pubmed-meshheading:17720533-Nerve Tissue Proteins, pubmed-meshheading:17720533-Neurons, Afferent, pubmed-meshheading:17720533-Nociceptors, pubmed-meshheading:17720533-RNA, Messenger, pubmed-meshheading:17720533-Receptors, G-Protein-Coupled, pubmed-meshheading:17720533-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17720533-Sodium Channels, pubmed-meshheading:17720533-TRPV Cation Channels
pubmed:year
2007
pubmed:articleTitle
Nociceptors of dorsal root ganglion express proton-sensing G-protein-coupled receptors.
pubmed:affiliation
Department of Life Sciences, National Central University, JungLi, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't