Linked Life Data

17713553

Source:http://linkedlifedata.com/resource/pubmed/id/17713553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-11-21
pubmed:abstractText
In the present study, we report that media conditioned by polycythemia vera (PV) CD3+ cells promote BFU-E and CFU-Mk colony formation by both cord blood and PV peripheral blood CD34+ cells in the absence of exogenous cytokines and promoting megakaryocyte proplatelet formation. CD3+ cells constitutively produce elevated levels of IL-11, while stimulation with the addition of phytohemagglutinin (PHA) increased GM-CSF levels in most of the patients with PV. Anti-IL-11-neutralizing antibody partially inhibited the formation of BFU-E and CFU-Mk colonies promoted by PV CD3+ cell-conditioned media. Although IL-11 is not produced by normal T cells, real-time PCR and flow cytometric analysis showed that IL-11 was upregulated in the CD3+ cells of most PV patients as compared to normal CD3+ cells. In addition, a greater percentage of BFU-E colonies formed by PV CD34+ cells in the presence of PV CD3+ cell-conditioned media alone were JAK2V617F-positive as compared with that induced by EPO. We conclude that dysregulated production of soluble growth factor(s), including IL-11 and GM-CSF by PV T cells, contributes to the in vitro formation of erythroid colonies in the absence of exogenous cytokines by PV CD34+ cells and likely plays a role in sustaining hematopoiesis in PV.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1476-5551
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2433-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17713553-Cells, Cultured, pubmed-meshheading:17713553-Colony-Forming Units Assay, pubmed-meshheading:17713553-Culture Media, Conditioned, pubmed-meshheading:17713553-Erythroid Precursor Cells, pubmed-meshheading:17713553-Erythropoietin, pubmed-meshheading:17713553-Fetal Blood, pubmed-meshheading:17713553-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:17713553-Hematopoiesis, pubmed-meshheading:17713553-Hematopoietic Cell Growth Factors, pubmed-meshheading:17713553-Humans, pubmed-meshheading:17713553-Interleukin-11, pubmed-meshheading:17713553-Janus Kinase 2, pubmed-meshheading:17713553-Megakaryocytes, pubmed-meshheading:17713553-Mutation, Missense, pubmed-meshheading:17713553-Phytohemagglutinins, pubmed-meshheading:17713553-Polycythemia Vera, pubmed-meshheading:17713553-T-Lymphocytes
pubmed:year
2007
pubmed:articleTitle
T cells from patients with polycythemia vera elaborate growth factors which contribute to endogenous erythroid and megakaryocyte colony formation.
pubmed:affiliation
Division of Hematology/Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural