Source:http://linkedlifedata.com/resource/pubmed/id/17705180
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2007-8-23
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| pubmed:abstractText |
The S RNA genome segment of hantaviruses carried by Arvicolinae and Sigmodontinae rodents encodes the nucleocapsid (N) protein and has an overlapping (+1) open reading frame (ORF) for a putative nonstructural protein (NSs). The aim of this study was to determine whether the ORF is functional. A protein corresponding to the predicted size of Tula virus (TULV) NSs was detected using coupled in vitro transcription and translation from a cloned S segment cDNA, and a protein corresponding to the predicted size of Puumala virus (PUUV) NSs was detected in infected cells by Western blotting with an anti-peptide serum. The activities of the interferon beta (IFN-beta) promoter, and nuclear factor kappa B (NF-kappaB)- and interferon regulatory factor-3 (IRF-3) responsive promoters, were inhibited in COS-7 cells transiently expressing TULV or PUUV NSs. Also IFN-beta mRNA levels in IFN-competent MRC5 cells either infected with TULV or transiently expressing NSs were decreased. These data demonstrate that Tula and Puumala hantaviruses have a functional NSs ORF. The findings may explain why the NSs ORF has been preserved in the genome of most hantaviruses during their long evolution and why hantavirus-infected cells secrete relatively low levels of IFNs.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocapsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0146-6615
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 2007 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
79
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1527-36
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17705180-Amino Acid Sequence,
pubmed-meshheading:17705180-Animals,
pubmed-meshheading:17705180-COS Cells,
pubmed-meshheading:17705180-Cell Line,
pubmed-meshheading:17705180-Cercopithecus aethiops,
pubmed-meshheading:17705180-Down-Regulation,
pubmed-meshheading:17705180-Hantavirus,
pubmed-meshheading:17705180-Hantavirus Infections,
pubmed-meshheading:17705180-Hemorrhagic Fever with Renal Syndrome,
pubmed-meshheading:17705180-Humans,
pubmed-meshheading:17705180-Interferon Regulatory Factor-3,
pubmed-meshheading:17705180-Interferon-beta,
pubmed-meshheading:17705180-Molecular Sequence Data,
pubmed-meshheading:17705180-NF-kappa B,
pubmed-meshheading:17705180-Nucleocapsid Proteins,
pubmed-meshheading:17705180-Open Reading Frames,
pubmed-meshheading:17705180-Promoter Regions, Genetic,
pubmed-meshheading:17705180-Puumala virus,
pubmed-meshheading:17705180-Sequence Alignment,
pubmed-meshheading:17705180-Viral Nonstructural Proteins
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| pubmed:year |
2007
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| pubmed:articleTitle |
Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter.
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| pubmed:affiliation |
Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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