Linked Life Data

17699562

Source:http://linkedlifedata.com/resource/pubmed/id/17699562

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-10-3
pubmed:abstractText
Previous data from spaceflight studies indicate that injured muscle and bone heal slowly and abnormally compared with ground controls, strongly suggesting that ligaments or tendons may not repair optimally as well. Thus the objective of this study was to investigate the biochemical and molecular gene expression of the collagen extracellular matrix in response to medial collateral ligament (MCL) injury repair in hindlimb unloaded (HLU) rodents. Male rats were assigned to 3- and 7-wk treatment groups with three subgroups each: sham control, ambulatory healing (Amb-healing), and HLU-healing groups. Amb- and HLU-healing animals underwent bilateral surgical transection of their MCLs, whereas control animals were subjected to sham surgeries. All surgeries were performed under isoflurane anesthesia. After 3 wk or 7 wk of HLU, rats were euthanized and MCLs were surgically isolated and prepared for molecular or biochemical analyses. Hydroxyproline concentration and hydroxylysylpyridinoline collagen cross-link contents were measured by HPLC and showed a substantial decrement in surgical groups. MCL tissue cellularity, quantified by DNA content, remained significantly elevated in all HLU-healing groups vs. Amb-healing groups. MCL gene expression of collagen type I, collagen type III, collagen type V, fibronectin, decorin, biglycan, lysyl oxidase, matrix metalloproteinase-2, and tissue inhibitor of matrix metalloproteinase-1, measured by real-time quantitative PCR, demonstrated differential expression in the HLU-healing groups compared with Amb-healing groups at both the 3- and 7-wk time points. Together, these data suggest that HLU affects dense fibrous connective tissue wound healing and confirms previous morphological and biomechanical data that HLU inhibits the ligament repair processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0363-6119
pubmed:author
pubmed:issnType
Print
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R1552-60
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:17699562-Adaptation, Physiological, pubmed-meshheading:17699562-Animals, pubmed-meshheading:17699562-Collagen Type I, pubmed-meshheading:17699562-Collagen Type III, pubmed-meshheading:17699562-Collagen Type V, pubmed-meshheading:17699562-Decorin, pubmed-meshheading:17699562-Down-Regulation, pubmed-meshheading:17699562-Extracellular Matrix, pubmed-meshheading:17699562-Extracellular Matrix Proteins, pubmed-meshheading:17699562-Fibronectins, pubmed-meshheading:17699562-Gene Expression Regulation, pubmed-meshheading:17699562-Hindlimb Suspension, pubmed-meshheading:17699562-Ligaments, pubmed-meshheading:17699562-Male, pubmed-meshheading:17699562-Matrix Metalloproteinase 2, pubmed-meshheading:17699562-Protein-Lysine 6-Oxidase, pubmed-meshheading:17699562-Proteoglycans, pubmed-meshheading:17699562-Rats, pubmed-meshheading:17699562-Rats, Sprague-Dawley, pubmed-meshheading:17699562-Time Factors, pubmed-meshheading:17699562-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:17699562-Up-Regulation, pubmed-meshheading:17699562-Wound Healing
pubmed:year
2007
pubmed:articleTitle
Temporal extracellular matrix adaptations in ligament during wound healing and hindlimb unloading.
pubmed:affiliation
Connective Tissue Physiology Laboratory, Department of Health and Human Performance, Univ. of Houston, N207 D Engineering Bldg. 1, Houston, TX 77204-4006, USA. ddam@uh.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.