Linked Life Data

17681543

Source:http://linkedlifedata.com/resource/pubmed/id/17681543

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-9-3
pubmed:databankReference
pubmed:abstractText
Glucosamine 6-phosphate (GlcN-6-P) synthase is an ubiquitous enzyme that catalyses the first committed step in the reaction pathway that leads to formation of uridine 5'-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc), a precursor of macromolecules that contain amino sugars. Despite sequence similarities, the enzyme in eukaryotes is tetrameric, whereas in prokaryotes it is a dimer. The activity of eukaryotic GlcN-6-P synthase (known as Gfa1p) is regulated by feedback inhibition by UDP-GlcNAc, the end product of the reaction pathway, whereas in prokaryotes the GlcN-6-P synthase (known as GlmS) is not regulated at the post-translational level. In bacteria and fungi the enzyme is essential for cell wall synthesis. In human the enzyme is a mediator of insulin resistance. For these reasons, Gfa1p is a target in anti-fungal chemotherapy and in therapeutics for type-2 diabetes. The crystal structure of the Gfa1p isomerase domain from Candida albicans has been analysed in complex with the allosteric inhibitor UDP-GlcNAc and in the presence of glucose 6-phosphate, fructose 6-phosphate and an analogue of the reaction intermediate, 2-amino-2-deoxy-d-mannitol 6-phosphate (ADMP). A solution structure of the native Gfa1p has been deduced using small-angle X-ray scattering (SAXS). The tetrameric Gfa1p can be described as a dimer of dimers, with each half similar to the related enzyme from Escherichia coli. The core of the protein consists of the isomerase domains. UDP-GlcNAc binds, together with a metal cation, in a well-defined pocket on the surface of the isomerase domain. The residues responsible for tetramerisation and for binding UDP-GlcNAc are conserved only among eukaryotic sequences. Comparison with the previously studied GlmS from E. coli reveals differences as well as similarities in the isomerase active site. This study of Gfa1p focuses on the features that distinguish it from the prokaryotic homologue in terms of quaternary structure, control of the enzymatic activity and details of the isomerase active site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
372
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
672-88
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17681543-Amino Acid Sequence, pubmed-meshheading:17681543-Bacterial Proteins, pubmed-meshheading:17681543-Binding Sites, pubmed-meshheading:17681543-Candida albicans, pubmed-meshheading:17681543-Crystallography, X-Ray, pubmed-meshheading:17681543-Escherichia coli, pubmed-meshheading:17681543-Fructosephosphates, pubmed-meshheading:17681543-Glucose-6-Phosphate, pubmed-meshheading:17681543-Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing), pubmed-meshheading:17681543-Isomerases, pubmed-meshheading:17681543-Ligands, pubmed-meshheading:17681543-Metals, pubmed-meshheading:17681543-Models, Molecular, pubmed-meshheading:17681543-Molecular Sequence Data, pubmed-meshheading:17681543-Protein Structure, Quaternary, pubmed-meshheading:17681543-Protein Structure, Secondary, pubmed-meshheading:17681543-Scattering, Small Angle, pubmed-meshheading:17681543-Solutions, pubmed-meshheading:17681543-Static Electricity, pubmed-meshheading:17681543-Substrate Specificity, pubmed-meshheading:17681543-Uridine Diphosphate N-Acetylglucosamine, pubmed-meshheading:17681543-X-Ray Diffraction
pubmed:year
2007
pubmed:articleTitle
The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans.
pubmed:affiliation
Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul. Noskowskiego 12/14, 61-704 Poznan, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't