Linked Life Data

17660945

Source:http://linkedlifedata.com/resource/pubmed/id/17660945

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-10-30
pubmed:abstractText
An iron-sulfur cluster assembly protein, IscU, is encoded by the operon iscSUA in Acidithiobacillus ferrooxidans. The gene of IscU was cloned and expressed in Escherichia coli. The protein was purified by one-step affinity chromatography to homogeneity. The protein was in apo-form, the [Fe(2)S(2)] cluster could be assembled in apoIscU with Fe(2+) and sulfide in vitro, and in the presence of IscA and IscS, the IscU could utilize L: -cysteine and Fe(2+) to synthesize [Fe(2)S(2)] cluster in the protein. Site-directed mutagenesis for the protein revealed that Cys37, Asp39, Cys63 and Cys106 were involved in ligating with the [Fe(2)S(2)] cluster.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0141-5492
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1965-72
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Expression, purification and characterization of an iron-sulfur cluster assembly protein, IscU, from Acidithiobacillus ferrooxidans.
pubmed:affiliation
Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't