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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2007-8-30
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pubmed:abstractText |
Our functional mapping study of murine gammaherpesvirus 68 (MHV-68, or gammaHV-68) revealed that a mutant harboring a transposon at the ORF49 locus (ORF49(null)) evidenced a highly attenuated in vitro growth. ORF49 resides adjacent to and in an opposite direction from RTA, the primary switch of the gammaherpesvirus life cycle. A FLAG-tagged ORF49 protein was able to transcomplement ORF49(null), and a revertant of ORF49(null) restored its attenuated growth to a level comparable to that of the wild type. The FLAG-tagged ORF49 protein promoted the ability of RTA to activate downstream target promoters and enhanced virus replication from the ORF50(null) virus in the presence of RTA. Furthermore, ORF49 enhanced wild-type virus replication by increasing the RTA transcript levels. Our data indicate that ORF49 may perform an important function in MHV-68 replication in cooperation with RTA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10194235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10400733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10448140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10458986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10516043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10644377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10729142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10856251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-10859385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-11086135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-11313012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-11313013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-11333906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-11533188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-12970434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-15113878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-15163752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-15709045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-15738413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-16439562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-16501115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-1660209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-2152819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-2987963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-3028777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-8799177,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17634244-9724796
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9870-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17634244-Animals,
pubmed-meshheading:17634244-Cercopithecus aethiops,
pubmed-meshheading:17634244-Cricetinae,
pubmed-meshheading:17634244-DNA Transposable Elements,
pubmed-meshheading:17634244-Genetic Complementation Test,
pubmed-meshheading:17634244-Humans,
pubmed-meshheading:17634244-Immediate-Early Proteins,
pubmed-meshheading:17634244-Mice,
pubmed-meshheading:17634244-Mutagenesis, Insertional,
pubmed-meshheading:17634244-Open Reading Frames,
pubmed-meshheading:17634244-Protein Binding,
pubmed-meshheading:17634244-Receptors, Cell Surface,
pubmed-meshheading:17634244-Rhadinovirus,
pubmed-meshheading:17634244-Trans-Activators,
pubmed-meshheading:17634244-Vero Cells,
pubmed-meshheading:17634244-Viral Proteins,
pubmed-meshheading:17634244-Viral Regulatory and Accessory Proteins,
pubmed-meshheading:17634244-Virus Replication
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pubmed:year |
2007
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pubmed:articleTitle |
The ORF49 protein of murine gammaherpesvirus 68 cooperates with RTA in regulating virus replication.
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pubmed:affiliation |
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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