Linked Life Data

17606302

Source:http://linkedlifedata.com/resource/pubmed/id/17606302

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-9-7
pubmed:abstractText
The conventional methods for producing recombinant adeno-associated virus (rAAV) rely on transient transfection of adherent mammalian cells. To gain acceptance and achieve current good manufacturing process (cGMP) compliance, clinical grade rAAV production process should have the following qualities: simplicity, consistency, cost effectiveness, and scalability. Currently, the only viable method for producing rAAV in large-scale, e.g. > or =10(16) particles per production run, utilizes baculovirus expression vectors (BEVs) and insect cells suspension cultures. The previously described rAAV production in 40 L culture using a stirred tank bioreactor requires special conditions for implementation and operation not available in all laboratories. Alternatives to producing rAAV in stirred tank bioreactors are single-use, disposable bioreactors, e.g. Wave. The disposable bags are purchased pre-sterilized thereby eliminating the need for end-user sterilization and also avoiding cleaning steps between production runs thus facilitating the production process. In this study, rAAV production in stirred tank and Wave bioreactors was compared. The working volumes were 10 L and 40 L for the stirred tank bioreactors and 5 L and 20 L for the Wave bioreactors. Comparable yields of rAAV, approximately 2E+13 particles per liter of cell culture were obtained in all volumes and configurations. These results demonstrate that producing rAAV in large scale using BEVs is reproducible, scalable, and independent of the bioreactor configuration.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-11027174, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-11390617, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-12427305, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-12432586, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-12545558, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-13679600, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-14625046, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-15488616, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-15903253, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-16035948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-16568890, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-16612615, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-16678231, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-16997013, http://linkedlifedata.com/resource/pubmed/commentcorrection/17606302-17010669
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Production of recombinant adeno-associated vectors using two bioreactor configurations at different scales.
pubmed:affiliation
Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Intramural