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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1992-2-6
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pubmed:abstractText |
In a prospective series of patients with transitional-cell bladder carcinomas, DNA ploidy and ABH antigen reactivity were determined in both frozen tissue and formalin-fixed paraffin blocks. ABH antigen measurement in frozen tissue was hampered by methodological problems, especially insufficient morphology, which made quantification difficult. In only 38/55 (69%) cases was the same ABH reactivity found. The knowledge of secretor status was not helpful in the interpretation of these results. In 53/59 (90%) cases the same DNA ploidy was achieved, resulting in good correlation between flow cytometric DNA measurements in formalin-fixed and frozen tissue. The deviations occurred mainly in cases exhibiting a tetraploid DNA profile. DNA assessments using image cytometry on imprints of fresh tissue was a rapid and reliable method in our hands, with identical results being obtained in 32/36 (89%) cases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0300-5623
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
357-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1759329-ABO Blood-Group System,
pubmed-meshheading:1759329-Carcinoma, Transitional Cell,
pubmed-meshheading:1759329-DNA, Neoplasm,
pubmed-meshheading:1759329-Humans,
pubmed-meshheading:1759329-Immunoenzyme Techniques,
pubmed-meshheading:1759329-Prospective Studies,
pubmed-meshheading:1759329-Urinary Bladder,
pubmed-meshheading:1759329-Urinary Bladder Neoplasms
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pubmed:year |
1991
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pubmed:articleTitle |
Determination of DNA ploidy and ABH antigen reactivity in both frozen and formalin-fixed bladder tumor tissue.
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pubmed:affiliation |
Department of Urology, Uppsala University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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